In this project, the IMProGlyco team are developing an exciting new strategy to engineer glycans in mammalian cells. This approach will be used to tailor the production of individual proteins and cell lines, changing the pattern of observed glycoforms to modulate their activity and hence pharmacological properties.
Secreted and cell-surface proteins in human cells are often heavily modified by N- and O-linked glycosylation. These modifications are introduced by the combined, sequential action of multiple individual enzymes. The specificity and selectivity of these enzymes together with variation in protein production rates and other factors mean that individual proteins and cells can exhibit complex patterns of glycoforms which are challenging to both analyse and engineer. In this project, the IMProGlyco approach will be to profile, understand and re-engineer these glycoforms.
Please direct any enquiries to our project adminstration team: IMProGlyco@leeds.ac.uk
The project is funded through the EIC Pathfinder Open scheme. (101186774 – IMProGlyco)
